SPDEF acts as a transcription factor by binding to the promoter regions of genes, thus we analyzed public SPDEF ChIP-seq data (GSE86957) to identify its potential target genes.27 We also performed Spearman correlation analysis using TCGA-HNSC cohort to determine genes that were positively correlated with SPDEF in HNSCC. This evidence concerns the gene SPDEF and head and neck squamous cell carcinoma.