Western blotting results showed sh-Hsp90 and sh-Hsp90+vector significantly inhibited Hsp90, HER2, p-HER2, p-AKT and PD-L1 expressions as compared to the control and sh-NC, while sh-Hsp+PD-L1 only restored PD-L1 expression (Fig. 6F), indicating PD-L1 was the downstream of Hsp90/HER2/PI3K/AKT in HPV16+ cervical cancer. This evidence concerns the gene AKT1 and cervical carcinoma.