Next, in order to further interrogate the dynamics of infection in the context of pooled libraries of AAV variants, and to determine whether the presence of other AAV variants impedes infection of library members, HEK293 cells grown in vitro were infected with either AAV2 alone, or with a pool containing 4 AAV’s (AAV2, AAV8, AAV9 and K912), or with a pool containing 16 AAV’s (AAV2, AAV8, AAV9, K912, AAV1, AAVrh10, AAV2-4YF, AAV2-4YF-T491V, AAV8-2YF, AAV9-2YF, K91, K916, K94, NHP9, NHP26, and SCH NHP9/26) (Figure 5B). This evidence concerns the gene NF2 and infection.