TNF and osteosarcoma: With the time course on U937 cells in hand, we selected the 15-min time point to interrogate TNF signaling in other cell lines (A549 (adenocarcinomic alveolar basal epithelial cell line), HT29 (colorectal adenocarcinoma cell line), and U2OS (osteosarcoma cell line)) and another relevant primary cell system, murine bone marrow-derived macrophages (BMDMs).