However, Gpr83−/− mice were protected from diet-induced obesity and glucose intolerance, and plasma levels of acyl-ghrelin and des-acyl-ghrelin were unaffected when fed a high-fat diet for 18 weeks, indicating that GPR83 likely has ghrelin-independent roles in appetite regulation. This evidence concerns the gene GHRL and obesity due to melanocortin 4 receptor deficiency.