To confirm whether the observed pattern of communication between RBCs and cardiomyocytes after IR is mediated by RBC-EVs and not a result of cell-fusion or ectopic expression of Cre after MI (40, 48), we performed IR on recipient mTmG mice injected with Cre-containing RBC-EVs (harvested from EpoR-Cre RBCs) during reperfusion (Fig S6A). Here, EPOR is linked to myocardial infarction.