Through chromatin immunoprecipitation-qRT-PCR (ChIP-qRT-PCR) and reporter gene assay, we further confirmed that UBTF promoted melanoma cell growth and cell cycle progression by facilitating G-protein-coupled receptor kinase-interacting protein 1 (GIT1) transcription, thereby activating MEK1/2-ERK1/2 signalling pathways. The gene discussed is MAP2K1; the disease is melanoma.