This study’s main objective is to assess the prognostic role of TFEB, CARM1, SIRT1, and Beclin-1 in chemo-treated breast carcinoma and compare the TFEB, CARM1, SIRT1, and Beclin-1 expression with the methylation of PITX2 in breast cancer treated with chemotherapy. The gene discussed is TFEB; the disease is breast carcinoma.