In this study, we aimed to explore the regulatory mechanism of bone marrow mesenchymal stem cell-derived extracellular vesicles (BMSCs-EVs)-mediated miR-23b in lipopolysaccharide (LPS)-induced spinal cord injury (SCI) in vivo and in vitro by negatively regulating the TLR4/NF-κB pathway. This evidence concerns the gene TLR4 and spinal cord injury.