We therefore examined the subcellular localisation of EML4‐ALK V1 and V3 by fixed‐cell imaging in several cell lines: NSCLC patient‐derived cancer cell lines harbouring endogenous EML4‐ALK V1 (H3122) or EML4‐ALK V3 (H2228), a non‐transformed human lung epithelial cell line (Beas2B) expressing EML4‐ALK V1 or V3 in a doxycycline‐inducible manner and HEK293 cells overexpressing YFP‐EML4‐ALK V1 or V3. Here, EML4 is linked to cancer.