Several studies have investigated the efficacies of subunit cocktail vaccines comprising ROP4 along with various other antigens such as ROP2, GRA4, SAG1, and MAG1 which significantly reduced the cyst burden in mice challenge-infected with T. gondii DX strain, a low-virulent type II strain similar to ME49 (Dziadek et al., 2009; Dziadek et al., 2011; Dziadek et al., 2012; Gatkowska et al., 2018). Here, GPAT3 is linked to cyst.