Dividing all cells into nine clusters by the R software with Seurat package based on the expressions of marker genes (Figure 7G and Supplementary Figure S3), the distribution of CDCA2 and P53 in HCC cells was highly consistent, and both were predominantly expressed in cells in cluster 7 (Figure 7H, Supplementary Figure S4 and Supplementary Tables S16, S17). This evidence concerns the gene TP53 and hepatocellular carcinoma.