In addition, for patients with central lung mass, moderate to large pleural effusion ipsilaterally, extensive infiltrating lesions, rapid disease progression and poor response to initial therapy, anti-NUT monoclonal antibody immunohistochemical staining should be performed as soon as possible, if necessary, combined with FISH (using the NUTM1 dual-color translocation rearrangement probes and the sensitivity of combining FISH with C52 IHC for diagnosing NUT carcinoma can reach 100%), NGS or whole genome sequencing. This evidence concerns the gene NUTM1 and Pleural effusion.