To examine the significance of inflammatory TME condition in CRC progression, we first investigated the proliferation capacity of HCT116 cells in 3D-alginate cultures alone (basal control) or co-cultured with MRC-5 fibroblasts and T-lymphocytes (multicellular proinflammatory TME cultures) and/or treated with TNF-β and/or with Calebin A (Figures 1A, B), by the MTT assay, as described in Materials and Methods. This evidence concerns the gene LTA and colorectal carcinoma.