As demonstrated in Figure 3, multicellular proinflammatory TME with T-lymphocytes or with TNF-β by itself prompted and significantly increased the number of colonosphere formations (Figures 3A, C) and invasion (Figures 3B, D) in HCT116 cells compared to that in basal control cultures, suggesting the pivotal role of multicellular proinflammatory TME cultures-mediated malignant potential of CRC cells. This evidence concerns the gene LTA and colorectal carcinoma.