To our knowledge, we have shown for the first time that the cytokine TNF-β-TME mediates proinflammatory signals in alginate HCT116 3D-alginate, similar to T-lymphocytes multicellular TME, which underlines the important role of this cytokine in the proinflammatory progression of CRC cells. The gene discussed is LTA; the disease is colorectal carcinoma.