Using the ChIP Seq bam files, we visualized the exact genomic location of DVL-1 binding (blue peak) at various cancer-associated genes not previously designated as Wnt target genes such as TRIO, COL5A1, EXD3, OR4A47, GLDN, and EFCAB6 compared to IgG control (red peak) using IGV (Figure 4A, Supplementary Figure 2). This evidence concerns the gene COL5A1 and cancer.