As shown in Figure 3C and E, the expression of Mcl-1 protein in AML cells increased when ABT-199 was treated alone, and metformin alone could significantly reduce the expression of Mcl-1, and when the two drugs were used in combination, the expression level of Mcl-1 was significantly lower than that of ABT-199 alone (Figure 3D, F). This evidence concerns the gene MCL1 and acute myeloid leukemia.