To this end, we knocked down MBNL2 expression by using shRNA, or overexpressed His-MBNL2 in MDA-MB-231 breast cancer cells, A549 non-small-cell lung cancer cells, and HepG2 liver cancer cells, the knock-down or knock-in efficiency were verified by qRT-PCR and western blot (Figure 2C). The gene discussed is MBNL2; the disease is liver cancer.