To finally ascertain whether upregulation of H2AFX is responsible for the inhibition of miR-328-3p-mediated migration and invasion of HNSCC, Tca8113 cells were treated with H2AFX overexpressed plasmid and empty vector following the transfection with miR-328-3p-mimic and NC. Here, H2AX is linked to head and neck squamous cell carcinoma.