E2F1 and glioma: Luciferase reporter assay showed that both the miR-320a mimic and sh-FGF14-AS2 reduced the luciferase activity of E2F1-WT but not E2F1-MUT (Fig. 5B, C). The expression of E2F1 in glioma tissues and glioma cell lines was measured, and the results showed that E2F1 was upregulated in glioma tissues and cell lines compared with normal brain tissues and normal human astrocytes, respectively (Fig. 5D, E).