The ssGSEA method was used to further evaluate the immune-cell infiltration status of TCGA colorectal cancer transcriptome, and the results suggested that neutrophils, macrophage M1 cells, T cells, and CD4 memory resting cells were enriched in the low-risk group, while M0 cells and T cell regulatory cells were more common in the high-risk group, numerous studies have shown that dense infiltration of T cells, especially cytotoxic CD8 T cells, and high density of M1 macrophages may be associated with acute inflammation, suggesting a good prognosis (Fuchs et al., 2019; Marcelis et al., 2020). This evidence concerns the gene CD4 and colorectal cancer.