Four cancer cell lines (MCF-7 human breast adenocarcinoma cells, A549 human alveolar adenocarcinoma cells, Caco-2 human epithelial colorectal adenocarcinoma cells, and HCT116 human colorectal carcinoma cells) with EGF+ phenotype were selected to test for cell proliferative effects of EGF content in EBN using MTT assay. This evidence concerns the gene EGF and cancer.