We found that compared with the model and mir-30b-5p non-loaded NPs, treatment with mir-30b-5p-loaded NPs significantly reduced the levels of cardiac hypertrophy markers (ANP, BNP, and β-MHC), suggesting that mir-30b-5p-loaded NPs have a therapeutic effect on cardiac hypertrophy. The gene discussed is NPPB; the disease is cardiac hypertrophy.