We found that BO treatment when accompanied with the regulation on amino acid metabolism promoted the phosphorylation of S6 and 4E-BP1, rather than those of PI3K, Akt, or mTOR, indicating that mTOR was activated by BO in an amino acid–regulated form, which correspondingly restrained autophagy to suppress tumor growth. This evidence concerns the gene AKT1 and bronchiolitis obliterans syndrome.