In both HD Family and IsoHD MAP2 positive neurons, RAN nuclear staining intensity was comparable between control and HD (Supplementary Figure 4A), whereas increased cytoplasmic intensity was apparent in 58Q, 69Q, 75Q, and IsoHD 81Q compared to control lines (Supplementary Figure 4B) suggesting an increased concentration of RAN within the cytoplasm. This evidence concerns the gene RAN and Huntington disease.