EA may relieve VH by reducing the mast cells number and the expression of PAR-2, substance P, CGRP, TRPV1, Toll-like receptor 4, and tryptase proteins in the colon tissue of IBS rats (Deng et al., 2018; Yang et al., 2019; Chen et al., 2021). This evidence concerns the gene F2RL1 and irritable bowel syndrome.