To delve deeper into the influences of LINC01094, miR-340-5p and E2F3 on BC cell proliferation, cell cycle and apoptosis, we transfected LINC01094 overexpression plasmids, LINC01094 overexpression plasmids + miR-340-5p mimics and LINC01094 overexpressoin plasmids + miR-340-5p mimics + E2F3 overexpression plasmids into MDA-MB-231 cells, and transfected si-LINC01094-1, si-LINC01094-1 + miR-340-5p inhibitors and si-LINC01094-1 + miR-340-5p inhibitors + si-E2F3 into MCF-7 cells. This evidence concerns the gene LINC01094 and breast cancer.