To examine whether WNT1 downregulation was due to direct binding with the miRNA, we then conducted luciferase expression assays for comparing WNT1 expression between CRC cells expressing the wild-type 3ʹ-UTR sequence of WNT1 or a sequence mutated within the putative binding region (figure 3f). This evidence concerns the gene WNT1 and colorectal carcinoma.