We further evaluated whether RBP‐Jκ induced colon cancer cell migration and invasion through TGF‐β1 expression in TAMs by establishing six different coculture systems: i) RKO‐NC coculture with M2‐NC2; ii) RKO‐shR coculture with M2‐NC2; iii) RKO‐shR coculture with M2‐T; iv) SW480‐NC coculture with M2‐NC1; v) SW480‐R coculture with M2‐NC1; and vi) SW480‐R coculture with M2‐siT, for migration, invasion and wound healing assays. The gene discussed is TGFB1; the disease is malignant colon neoplasm.