Both BNIP3 and BNIP3L were strongly induced by hypoxia such that at 8 h following the switch to 1% oxygen, both proteins were maximally expressed and their levels sustained through 16 h of hypoxia in both U2OS and Saos2 osteosarcoma cells (Fig. 5a, U2OS; Fig. 5b, Saos2—lanes 1–4). This evidence concerns the gene BNIP3 and osteosarcoma.