CHD1L and hepatocellular carcinoma: As expected, knockdown of ZKSCAN3 induced a higher ratio of yellow autophagosomes puncta and red autolysosomes puncta (Fig. 5C, D), indicating that the inhibited autophagic flux by depletion of CHD1L was reversed after downregulation of ZKSCAN3. To determine whether ZKSCAN3 is essential in CHD1L-induced HCC cell migration, the effects of ZKSCAN3 downregulation on HCC cell migration after CHD1L deletion were examined in transwell assay and wound-healing assay.