Transcript levels of NLRC5, the master regulator of HLA class I genes, were significantly higher in TOs compared with TSC-2D and TSC-3D, indicating that mRNA expression did not correlate with the functional expression levels (protein expression in Fig. 1 and Fig. S1) (Neerincx et al., 2012). This evidence concerns the gene NLRC5 and tuberous sclerosis.