TWIST1 and neoplasm: Notably, it has been widely recognized that the process of EMT facilitates tumor cells proliferation, motility, and invasion during tumor development, including gastric tumorigenesis and progression (Christiansen and Rajasekaran, 2006; Natalwala et al., 2008; Peng et al., 2014) we next analyzed the protein levels of EMT markers including epithelial marker E-Cadherin, mesenchymal marker Vimentin as well as EMT promoting transcription factor Twist.