This retrospective study aimed to evaluate the usefulness of novel MFC data analysis software for comparing the immunophenotypic profile of myeloid hematopoietic precursors (myHPCs) from normal bone marrow (NBM) samples with that of leukemic blasts from AML cases carrying recurrent genetic abnormalities, such as t(8;21), t(15;17), inv(16), and MLL (KMT2A) gene alterations. Here, KMT2A is linked to acute myeloid leukemia.