PCK1 and neoplasm: To further explore the mechanism by which OGT-mediated protein O-GlcNAcylation facilitates PCK1-KO cell migration and invasion, we screened for intracellular proteins that interact with OGT, using our previous immunoprecipitation assay coupled with mass spectrometry (IP-MS) data [16], and focused on lysine acetyltransferase 5 (KAT5), a histone acetyltransferase belonging to MYST family, that plays a significant role in tumor metastasis [27].