PCK1 and hepatocellular carcinoma: To further identify how PCK1 mediates KAT5 O-GlcNAcylation in HCC metastasis in vivo, WT and liver-specific Pck1-deletion (Pck1-LKO) mice were treated with 75 mg/kg of diethylnitrosamine (DEN) and fed with phenobarbital (PB) in a 0.05% diet to induce lung metastatic model of liver cancer [37].