Through FDR correction analysis, the methylation levels at 6 CpG sites within the ARHGAP40, STAT1, C5orf34, RDH12, and YAP1 genes were higher in the GDM group than in the control group, whereas those at 6 CpG sites within the HAPLN3, IFNGR2, YAP1, NFATC4, and DNAJB6 genes were lower in the GDM group than in the control group. This evidence concerns the gene YAP1 and gestational diabetes.