To determine the role of ROCK2 in regulating TPC’s BV supporting function, in vitro tube formation assay was performed by co-culturing HUVEC with NSCLC/HCC-derived TPC pre-treated with either placebo or ROCK inhibitor GSK 429286A in matrigel, indicating that pre-treatment with ROCK inhibitor significantly enhanced total tube length, branch point, and number (Fig. 5l–o). This evidence concerns the gene ROCK2 and hepatocellular carcinoma.