Doppler ultrasound analysis of microbubble perfusion revealed that depletion of HK2 in TPC enhanced A549 tumor blood flow and perfusion in mice as compared with scramble control group (Supplementary fig. 10r), while overexpression of ROCK2 in HK2-depleted TPC reduced the enhanced tumor blood flow and perfusion observed (Supplementary fig. 10r). This evidence concerns the gene ROCK2 and neoplasm.