We first stably knock-downed HK2, ROCK1, or ROCK2 expression in NSCLC/HCC-derived TPC, and also overexpressed ROCK2 in HK2-depleted TPC, which was confirmed by the RT-PCR and western blot analysis (Supplementary fig. 6h–k). The gene discussed is ROCK2; the disease is non-small cell lung carcinoma.