To confirm the role of pericyte-HK2 in modulating tumor vasculature and subsequent drug delivery, we subcutaneously co-injected A549/MHCC-LM9 (i.e. human HCC cell line) cells with either stable HK2-depleted TPC, stable ROCK2 overexpressing HK2-depleted TPC or scramble transfected TPC at 10:1 ratio into nude mice and then treated the tumor-bearing mice with placebo or doxorubicin. Here, ROCK2 is linked to hepatocellular carcinoma.