Ex vivo two photon imaging showed that LLC tumor blood vessel perfusion, vascular diameter, and doxorubicin delivery were all increased in mice treated combination group as compared with doxorubicin alone treated groups (Fig. 6l–n and Supplementary movie 1, 2), implying that HK2 inhibitor treatment induced MLC2-driven tumor vasculature remodeling. This evidence concerns the gene MYL2 and neoplasm.