To investigate the potential function of TRIM31 in breast cancer progression, we silenced TRIM31 expression in MCF7 and ZR-75-30 cells using lentivirus-delivered shRNAs (the sequences of sh-TRIM31 were shown in Table 2), and the level of TRIM31 was detected by real-time PCR and western blotting (Fig. 2a, b). Here, TRIM31 is linked to breast carcinoma.