Although anti-PD-1 antibody is expected to act on both Tregs and exhausted T cells, it profoundly exhibited antitumor effects in AMPKfl/flFoxp3-Cre mice compared to WT mice, suggesting that the increased anti-tumor immunity is due to the PD-1 blockade in Tregs, which is increased in the absence of AMPK signaling. The gene discussed is PDCD1; the disease is neoplasm.