Next, we assessed the tumor-antigen-specific cytotoxic T cell response using an in vivo CTL assay, and we observed that cytolytic activity of CD8+ T cells in the spleen and lymph nodes was significantly decreased in AMPKfl/flFoxp3-Cre mice (Fig. 1H), suggesting that AMPK supports the cytotoxic effect exerted by CD8+ T cells. This evidence concerns the gene PRKAA1 and neoplasm.