According to the FDR value of these pathways, ten pathways related to osteoporosis were screened out, including the estrogen signaling pathway, HIF-1 signaling pathway, VEGF signaling pathway, TNF signaling pathway, Ras signaling pathway, FoxO signaling pathway, MAPK signaling pathway, PI3K-Akt signaling pathway, Osteoclast differentiation and Inflammatory mediator regulation of TRP channels (Table 2). This evidence concerns the gene HIF1A and osteoporosis.