However, we observed that BMDMs induced to undergo hyperactivation following infection with ΔoatA mutant S. aureus exhibit reduced levels of total caspase-1 and GSDMD cleavage, suggesting that the regulated step in hyperactivation occurs at the level of caspase processing or complex assembly, rather than at the level of GSDMD pore formation. The gene discussed is GSDMD; the disease is infection.