ITGA2 and ovarian carcinoma: To experimentally validate the N-glycosylation sites of ITGA2, the protein lysate of ovarian cancer cells expressing hemagglutinin (HA)-tagged ITGA2 was immunoprecipitated with anti-HA magnetic beads, resolved by SDS-PAGE and the corresponding ITGA2 region was excised and subjected to in-gel digestion with either trypsin or chymotrypsin (FiguresS2A and S2B).