Further investigation of cell detachment-induced anoikis, a critical step of tumor cells with enhanced mesenchymal features or undergoing malignant transformation, showed a significant increase of apoptotic (Annexin V+/DAPI-)) and dead (Annexin V+/DAPI+) cells in KO and 10NQ cells (Figure 5E). This evidence concerns the gene ANXA5 and neoplasm.