The SPP1 concentration in plasma was quantified by ELISA, which showed SPP1 concentrations in patients with lymph node metastasis were significantly higher than in the non-metastatic group (Figure 8F, p < 0.05), Moreover, the ROC curve showed that the plasma SPP1 level had good efficiency for predicting early lymph node metastasis (AUC = 0.74, Figure 8G). This evidence concerns the gene SPP1 and metastatic malignant neoplasm in the lymph nodes.