To investigate the role of SESN2 in autophagy-mediated apoptosis, we treated SESN2-overexpressing and control cells with 3-MA (5 mM) and chemotherapeutic agents and observed that apoptosis of the SESN2-overexpressing HOS cells was increased and that the protective effect of SESN2 on osteosarcoma cells was decreased after 3-MA (5 mM) treatment (Figure 4D). Here, SESN2 is linked to osteosarcoma.