To further study the mechanism of oncogenic TRIB2 and its interaction with RFWD2 in lung cancer, performed immunoprecipitation and immunofluorescence experiments showed that TRIB2 colocalizes with RFWD2-related IκB-α to form a ternary complex and further affected IκB-α degradation by regulating its phosphorylation. The gene discussed is COP1; the disease is lung cancer.