Taking advantage of the linear dimension reduction algorithm with unsupervised principal components analysis (PCA), we found that patients with septic AKI had broader distribution in the up and right quadrants of the graph, while the healthy controls were mainly clustered in the down and left quadrants of the graph (Figure 1E & F), confirming the higher levels of NETs and Fn14 in patients with septic AKI than the healthy controls (Figure S2G & H). Here, TNFRSF12A is linked to acute kidney injury.