To ask whether the synergistic effects of combined NETs and Fn14 blockade against septic AKI could be recapitulated on a genetic background, the PAD4-deficient CMV-Cre; PAD4fl/fl mice and their PAD4fl/fl littermates were subjected to CLP, followed by intraperitoneal ITEM-2 injection at 24h after onset of sepsis for a further three days (Figure 4A). Here, PADI4 is linked to acute kidney injury.