As a complementary approach to evaluate the anti-septic AKI effects of combined NETs and Fn14 blockade, release of proinflammatory cytokines including high mobility group box 1 (HMGB1), interleukin-1β (IL-1β), tumor necrosis factor (TNF) and interleukin-6 (IL-6) were measured in CLP mice receiving both SIVE and ITEM-2 therapy. This evidence concerns the gene HMGB1 and acute kidney injury.