Because clinical cancer samples are devoid of fluorogenic markers, we then used our GFP-labeled cells, C3-labeled cells, and non-fluorogenic labeled cells in 3D-TSCs to test drug responses and to compare them to the following seven methods: MTT assay, PI staining, Hoechst 33342, LIVE/DEAD® viability/cytotoxicity kit, caspase-3/7 green detection reagent, NucRed live 647, and LysoTracker® deep red (Figure 3A-F and Figure S5-7). The gene discussed is CASP3; the disease is cancer.