Figure 6D showed PLOD3 protein presented with good diagnostic value between CRC and normal tissues. To further confirm the diagnostic value of PLOD3 in CRC patients, the expression of PLOD3 were analyzed by TMA-based IHC. we compared 160 CRC tumor tissues with paired adjacent normal tissues in a microarray, the representative IHC images of positive PLOD3 expression in tumor tissue and negative PLOD3 expression in normal tissue were shown (Figure 6E). Grossly, PLOD3 was overexpressed in tumor parts comparing to normal specimens (Figure 6F, p-value = 4.2e-14). The gene discussed is PLOD3; the disease is neoplasm.