Other results confirm that HFD has considerable adverse effects on skeletal muscle remodeling due to down-regulation of Col3a1 mRNA levels and MMP-2 activity, accompanied by up-regulation of Nfkb and Foxo1 mRNA levels in the offspring from trained fathers (TFO-HF vs. TFO-C). This evidence concerns the gene NFKB1 and hydrops fetalis.