Paternal RT did not modulate structural matrix proteins or matrix remodeling enzyme mRNA levels, like Col1 protein level and MMP-2 activity in the gastrocnemius muscle of offspring exposed to HFD, which could be related to similar Ctgf and Tgfb mRNA levels between the TFO-HF and SFO-HF groups. This evidence concerns the gene TGFB1 and hydrops fetalis.