Overall, these data showed that while β-ARs blockade, mainly β2-and β3-ARs and to a lesser extent β1-AR, is able to significantly influence the osteosarcoma tumor growth, the targeting of β2-and β3-ARs, but not of β1-AR, mitigated the established mechanical allodynia resulting from osteosarcoma tumor growth. The gene discussed is CTBP1; the disease is neoplasm.