In addition to commonly used NfL and pNfH, some studies also found potential values of other Nf subunits, i.e., NfM (Hu et al., 2002; Haggmark et al., 2014; Martinez-Morillo et al., 2014; Zucchi et al., 2018; Remnestal et al., 2020), INA (Martinez-Morillo et al., 2014) and PRPH (Finderlater, 2010; Liang et al., 2019; Sabbatini et al., 2021) as biomarkers in CSF or serum in neurological diseases or injuries. This evidence concerns the gene NEFL and nervous system disorder.